Aktivitas Antibakteri Ekstrak Bawang Hitam Variasi Waktu Aging Terhadap Pencegahan Dysbiosis Kulit Penyebab Jerawat

Antibacterial Activity of Black Garlic Extract Variations in Aging Time on Prevention of Skin Dysbiosis Causes Acne

Authors

  • Luthfia Hastiani Muharram Program Studi Bioteknologi Universitas Muhammadiyah Bandung
  • Fauzia Ningrum Syaputri Program Studi Farmasi Universitas Muhammadiyah Bandung
  • Wulan Pertiwi Program Studi Bioteknologi Universitas Muhammadiyah Bandung
  • Rizki Fika Saputri Program Studi Bioteknologi Universitas Muhammadiyah Bandung

DOI:

https://doi.org/10.25026/jsk.v4i2.1035

Keywords:

Skin Dysbiosis, Acne, Black Garlic, S. epidermidis, C. acnes

Abstract

Skin diseases, including acne, can be caused by a microbial imbalance (dysbiosis). The specific bacteria involved in acne dysbiosis are Cutibacterium (Propionibacterium) acnes and Staphylococcus epidermidis. Black garlic has better potential to be alternative antibiotic and chemical to curing ance and maintaining skin health, because it has antimicrobial potential and can inhibit the decrease in collagen deposition on the skin. These potentials can be alternative of antibiotics and chemicals to treat acne and maintain healthy skin. This study aimed to determine the antibacterial activity of black garlic extract against bacteria that cause acne dysbiosis. Garlic was aging with 3 variations of time (7, 14, and 21 days) then macerated with 70% alcohol and tested for antimicrobial activity to Cutibacterium acnes and Staphylococcus epidermidis with clindamycin as positive control. Black garlic extract provided inhibitory activity against Staphylococcus epidermidis and Cutibacterium acnes. The best recommendation time for aging garlic to prevent skin dysbiosis causes acne is 7 to 14 days.

References

[1] A. L. Byrd , Y. Belkaid Y and J. A. Segre, "The human skin microbiome.," Nat Rev Microbiol., vol. 16, pp. 143-155, Mar 2018.
[2] S. J. Grice EA, " The skin microbiome," Nat Rev Microbiol. , vol. 9(4), pp. 244-53, Apr 2011 .
[3] C. P. Tambolli , C. Neut, P. Desreumaux and J. Colomb, "Dysbiosis in inflammatory bowel disease," Gut, vol. 53(1), pp. 1-4, 2004.
[4] Y. B. Lee, E. J. Byun and H. S. Kim, "Potential Role of the Microbiome in Acne: A Comprehensive Review.," Journal of clinical medicine, vol. 8(7), p. 987, 2019.
[5] R. S, B. E, . D. L. T and L. H, "The role of the skin microbiota in acne pathophysiology," Br J Dermatol, pp. 691-699, 2019.
[6] N. I. F. Armandy, P. and . A. Pratiwi, "Uji Aktivitas Antibakteri sediaan Krim Antijerawat Ekstrak Etanol Terpurifikasi Daun Sirih (piper betle L) dengan Basis Vanishing Crem Terhadap Propionibacterium acne," Pharmauho, vol. 4, no. 2, 2018.
[7] N. S. Handayani , C. L. Bawono, P. D. Ayu and N. H. Pratiwi, "Isolasi Senyawa Polifeno Black garlic dan Uji Toksisitasnya Terhadapa larva udang ( Artemia salina L )," Jurnal Ilmu Kefarmasian Indonesia, vol. 16, no. 2, 2018.
[8] S. N and I. Y, "A Riview of component and Pharmacology activities of Black garlic," Traditional medicine journal, vol. 24, pp. 178-183, 2019.
[9] R. H. Aliya, I. T. Maulana and R. A. Kodir, "Perbandingan Aktivitas Antibakteri Bawang Putih (Allium sativum L) dengan Ekstrak Bawang Hitam terhadap Bakteri Penyebab Jerawat Propionibacterium acnes," Juornal of Pharmacey Science and practice, vol. 6, no. 2, 2020.
[10] B. Wasliati , W. Pangkahila and A. A. Wiraguna, "Krim ekstrak bawang hitam (Allium sativum L) Mencegah peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen dermis kulit tikus (Rattus norvegicus) Wastar Jantan yang dipapar sinar UV-B," CDK-278, vol. 46, no. 8, 2019.
[11] D. POM, "Ekstraksi," Ditjen POM Departemen Kesehatan RI, Jakarta, 2012.
[12] H. Jang, H. Lee, D. Yoon, D. Ji and J. Kim, "Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents," Food Sci Biotechnol,, vol. 27(1), p. 219–225, 2018.
[13] S. Aryal, "Mueller Hinton Agar (MHA) – Composition, Principle, Uses and Preparation," 25 October 2018. [Online]. Available: https://microbiologyinfo.com/mueller-hinton-agar-mha-composition-principle-uses-and-preparation/. [Accessed 22 March 2021].
[14] J. Mc.Farland, "Nephelometer: an instrument for media used for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines.," JAMA , vol. XLIX(14), p. 1176–1178., 1907.
[15] K. B. Hockett, "Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds," Journal of Visualized Experiment,, vol. 119, pp. 1-5, 2017.
[16] J. E. Hodge, "Dehydrated foods, chemistry of browning reactions in model systems," J Agric Food Chem, Vols. 1:928-43., 1953.
[17] Y. M. Jung , S. H. Lee, D. S. Lee , M. J. You , I. K. Chung and W. Cheon, "Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects," Nutr Res, vol. 31, pp. 387-96., 2011.
[18] M. J. Song K, " The influence of heating on the anticancer properties of garlic," J Nutr, , vol. 131(3s), pp. 1054S-7S., March 2001.
[19] H. Amagase , B. L. Petesch , H. Matsuura , S. Kasuga and Itaku, "Intake of garlic and its bioactive components," J Nutr, vol. 131, p. 955Se62S, 2001.
[20] H. Yuan, L. Sun, M. Chen and J. Wang, "The comparison of the contents of sugar, Amadori, and Heyns compounds in fresh and black garlic," J Food Sci., vol. 81, p. C1662e8., 2016.
[21] I. S. Choi , H. S. Cha and Y. S. Lee, "Physicochemical and Antioxidant Properties of Black Garlic.," Molecules, vol. 19, pp. 16811-23, 2014.
[22] A. W. Bauer, W. M. Kirby , J. C. Sherris and M. Turck, "Antibiotic susceptibility testing by a standardized single disk method," Am. J. Clin. Pathol., vol. 45, p. 493 496, 1966.
[23] N. C. f. C. L. Standards, Approved standard: M2-A8. Performance standards for antimicrobial disk susceptibility tests, Wayne, Pa: National Committee for Clinical Laboratory Standards, 2003.
[24] Y. Wang , M. S. Kao , J. Yu, S. Huang , S. Marito and R. L. Gallo, "A Precision Microbiome Approach Using Sucrose for Selective Augmentation of Staphylococcus epidermidis Fermentation against Propionibacterium acnes," Int J Mol Sci, vol. 17(11), p. E1870, 2016.
[25] M. A. Dagnelie , S. Corvec , M. Saint-Jean and V. N. Bourdès , "Decrease in Diversity of Propionibacterium acnes Phylotypes in Patients with Severe Acne on the Back," Acta Derm Venereol, vol. 98(2), p. 262–7, 2018.
[26] G. G. Aubin , M. E. Portillo , A. Trampuz and S. Corvec, "Propionibacterium acnes, an emerging pathogen: from acne to implant-infections, from phylotype to resistance," Med Mal Infect., vol. 44(6), p. 241–50, 2014.
[27] k. Nakase , H. Nakaminami , Y. Takenaka , N. Hayashi and M. Kawashima, "Relationship between the severity of acne vulgaris and antimicrobial resistance of bacteria isolated from acne lesions in a hospital in Japan," J Med Microbiol., vol. 5, p. 721–8, 2014.
[28] C. C. Zouboulis, E. Jourdan and M. Picardo, "Acne is an inflammatory disease and alterations of sebum composition initiate acne lesions," J Eur Acad Dermatol Venereol, vol. 28(5):, p. 527–32., 2014.
[29] M. J. Song K, "The influence of heating on the anticancer properties of garlic.," J Nutr, vol. 131(3s), pp. 1054S-7S, Mar 2001.
[30] H.-J. Jang, H.-J. Lee, D.-K. Yoon, D.-S. Ji, J.-H. Kim and C.-H. Lee, "Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents," Food Sci Biotechnol, vol. 27(1), p. 219–225, 2018.
[31] R. E. H. Aliya , I. T. Maulana and R. A. Kodir, "Perbandingan Aktivitas Antibakteri Bawang Putih (allium sativum l) dengan Ekstrak Bawang Hitam terhadap Bakteri Penyebab Jerawat Propionibacterium acnes," in Seminar Penelitian Sivitas Akademika Unisba, Bandung, Indonesia, 2020.
[32] N. d. Wildan, "Formulasi Krim dari Ekstrak Air Daun Alpukat (Persea Americana Mill) sebagai Sediaan Antiijerawat," Jurnal Penelitian Farmasi Indonesia, p. 2, 2019.
[33] A. Fatmawaty, N. A. Aisyah, M. Nisa and Kurisa , S, "Uji Aktivitas dan Formulasi krim Antijerawat dari beberapa bahan Alam," Prosedin rakernas dan pertemuan ilmiah tahunan, Ikatan Dokter Indonesia, 2016.

Downloads

Published

2022-04-30

How to Cite

Muharram, L. H., Syaputri, F. N. ., Pertiwi, W., & Saputri, R. F. (2022). Aktivitas Antibakteri Ekstrak Bawang Hitam Variasi Waktu Aging Terhadap Pencegahan Dysbiosis Kulit Penyebab Jerawat: Antibacterial Activity of Black Garlic Extract Variations in Aging Time on Prevention of Skin Dysbiosis Causes Acne. Jurnal Sains Dan Kesehatan, 4(2), 181–188. https://doi.org/10.25026/jsk.v4i2.1035