Penerapan Teknik Nanopresipitasi pada Nanoenkapsulasi Teofilin dengan Variasi Konsentrasi Polimer dan Surfaktan

Application of Nanoprecipitation Technique on Theophylline Nanoencapsulation with Variation of Polymer Concentration

Authors

  • Mar’atus Sholikhah Jurusan Farmasi, Poltekkes Kemenkes Palembang, Indonesia https://orcid.org/0000-0002-9110-8409
  • Rahayu Apriyanti Program Studi S1 Farmasi, Stikes Pelita Ibu Kendari, Indonesia
  • Sarmadi Sarmadi Jurusan Farmasi, Poltekkes Kemenkes Palembang, Indonesia

DOI:

https://doi.org/10.25026/jsk.v5i1.1521

Keywords:

Theophylline, nanoencapsulation, nanoprecipitation, nanoparticles

Abstract

Theophylline is a xanthine derivative used in the treatment of asthma that causes smooth muscle relaxation, especially bronchial smooth muscle dilation. So far, the pharmaceutical dosage forms of theophylline are very diverse, and most of them are intended for oral use. Oral administration of drugs is known to be prone to causing gastrointestinal disorders such as indigestion. In addition, its stability is also easy to change due to the influence of enzymes, pH, and microbial degradation. One of the most promising drug delivery systems is nanoencapsulation. The purpose of this study was to obtain the optimum formula for theophylline nanoencapsulation (NET) using an optimization system. Theophylline nanoencapsulation was prepared by applying various concentrations of PEG 6000 and SLS, starting from the smallest ratio of 1:1 to the largest ratio of 3:1. The characterization carried out on the five NET formulas included transmittance percentage, particle size, and polydispersity index (PDI). The selected optimal NET formula was then used to determine the zeta potential using Particle Size Analyzer. The ratio of 1.667:1 between PEG 6000 and SLS is the most optimal composition capable of producing NET with a particle size of 11.9 nm, a PDI of 0.12 µm, and a zeta potential of +18,87 mV.

Keywords: Nanoencapsulation, nanoparticles, nanoprecipitation, theophylline

Abstrak

Teofilin merupakan derivat xantin yang digunakan dalam terapi asma yang menyebabkan relaksasi otot polos, khususnya dilatasi otot polos bronkus. Sejauh ini bentuk sediaan farmasi teofilin sangat beragam dan sebagian besar ditujukan untuk penggunaan secara oral. Pemberian obat peroral diketahui rentan menyebabkan gangguan saluran cerna seperti indigesti. Selain itu, stabilitasnya juga mudah berubah karena adanya pengaruh enzim, pH, dan degradasi mikroba. Salah satu sistem penghantaran obat yang menjanjikan adalah nanoenkapsulasi. Tujuan dari penelitian ini adalah untuk memperoleh formula optimum dari nanoenkapsulasi teofilin (NET) menggunakan sistem optimasi. Nanoenkapsulasi teofilin dipreparasi dengan menerapkan variasi konsentrasi PEG 6000 dan SLS mulai dari perbandingan terkecil 1:1 hingga terbesar 3:1. Adapun karakterisasi yang dilakukan terhadap lima formula NET meliputi persentase transmitan, ukuran partikel, dan indeks polidispersitas (PDI). Formula optimum NET terpilih kemudian ditetapkan zeta potensialnya menggunakan Particle Size Analyzer. Perbandingan antara PEG 6000 dan SLS sebesar 1,667:1 merupakan komposisi paling optimum yang mampu menghasilkan NET dengan ukuran partikel 11,9 nm, PDI 0,12 µm, dan zeta potensial sebesar +18,87 mV.

Kata Kunci: Nanoenkapsulasi, nanopartikel, nanopresipitasi, teofilin

References

Sutradhar, K. B., & Amin, M, (2014). Nanotechnology in Cancer Drug Delivery and Selective Targeting, International Scholarly Research Notices, 1-12.

Parveen, S., & Sahoo, S. K, (2008). Polymeric Nanoparticles for Cancer Therapy, Journal of Drug Targeting, 16, (2), 108–123.

Buhecha, M. D., Lansley, A. B., Somavarapu, S., & Pannala, A. S, (2019). Development and Characterization of PLA Nanoparticles for Pulmonary Drug Delivery: Co-Encapsulation of Theophylline and Budesonide, A Hydrophilic and Lipophilic drug, Journal of Drug Delivery Science and Technology, 53, 1-13.

Akmarina, C. A, (2016). Aplikasi Kitosan Dalam Bidang Farmasetik, Farmaka, 14, (2), 318-330.

Akib, N. I., Ritonga, H., & Mahfuz, M, (2019). Preparasi Teofilin dalam Pembawa Vesikular Etosom untuk Penggunaan Transdermal, Jurnal Farmasi, Sains, dan Kesehatan, 5, (1), 25-28.

Rijal, M. A. S., Mikail, A., & Sari, R, (2010). Pengaruh pH Larutan Tripolifosfat Terhadap Karakteristik Fisik Serta Profil Pelepasan Mikropartikel Teofilin-Chitosan, Majalah Farmasi Airlangga, 8, (2), 26-30.

Popa N, Ovidlu N, Lenuta P, Catalina EL, Marcel IP. (2010). Hydrogels Based on Chitosan–Xanthan for Controlled Release of Theophylline. Journal of Materials Science: Materials in Medicine, 21, 1241-1248.

Jin, B., Zhou, X., Chen, C., Zhang, X., & Chen, S, (2015). Preparation, Characterization and In Vitro Evaluation of Theophylline Nanoparticles Prepared with Dextran-Conjugated Soy Protein, Tropical Journal of Pharmaceutical Research, 14, (8), 1323-1332.

Kadian, R, (2018). Nanoparticles: A promising drug delivery approach, Asian J Pharm Clin Res, 11, (1), 30-5.

Hapsari, R. B., Pranoto, Y., Murdiati, A., & Supriyanto, S, (2022). Optimasi Proses Nanopresipitasi pada Nanoenkapsulasi Ekstrak Kasar Daun Kakao (Theobroma cacao L.) Menggunakan Response Surface Methodology (RSM), agriTECH, 42, (1), 75-85.

Theodore. E.A., Mufeeda. T.,Puvitha. T.C., Manavalan. R., Venkatanarayanan. R, (2016). Formulation and Evaluation of Salbutamol Sulphate Nanoparticles, Asian Journal of Research in Biological And Pharmaceutical sciences, 4, (2), 80-88.

Thadakapally, R., Aafreen, A., Aukunuru, J., Habibuddin, M., & Jogala, S, (2016). Preparation and Characterization of PEG-Albumin-Curcumin Nanoparticles Intended to Treat Breast Cancer, Indian Journal of Pharmaceutical Sciences, 78, (1), 65.

Okore, V. C., Attama, A. A., Ofokansi, K. C., Esimone, C. O., & Onuigbo, E. B, (2011). Formulation and Evaluation of Niosomes, Indian Journal of Pharmaceutical Sciences, 73, (3), 323.

Masoud, E. A. M., Haji, B. S., & Mohammad, A. O, (2012). The Influence of Sucrose Ester Surfactants and Different Storage Conditions on the Preparation of a Novel Swietenia Macrophylla Oil Nanoemulsion, International Research Journal of Pharmacy, 3, (6), 199–207.

Artini, I. G. A, (2013). Peranan Nanopartikel Dalam Penatalaksanaan Kanker di Era Targeting Therapy, Indonesian Journal of Cancer, 7, (3).

Ferdiansyah, F., Heriyanto, H., Wijaya, C. H., & Limantara, L, (2017). Pengaruh Metode Nanoenkapsulasi terhadap Stabilitas Pigmen Karotenoid dan Umur Simpan Minyak dari Buah Merah (Pandanus conoideus L), Agritech, 37, (4), 369-376.

Masarudin, M. J., Cutts, S. M., Evison, B. J., Phillips, D. R., & Pigram, P. J, (2015). Factors Determining The Stability, Size Distribution, and Cellular Accumulation of Small, Monodisperse Chitosan Nanoparticles as Candidate Vectors For Anticancer Drug Delivery: Application to The Passive Encapsulation of Doxorubicin, Nanotechnology science and applications, 8, 67-80.

Laili, H. N., Winarti, L., & Sari, L. O. R. K, (2014). Preparasi dan Karakterisasi Nanopartikel Kitosan-Naringenin dengan Variasi Rasio Massa Kitosan-Natrium Tripolifosfat (Preparation and Caracterization of Naringenin-Chitosan Nanoparticles with Various Mass Ratio of Chitosan-Sodium Tripolyphosphat), Pustaka Kesehatan, 2, (2), 308-313.

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Published

2023-02-28

How to Cite

Sholikhah, M., Apriyanti, R., & Sarmadi, S. (2023). Penerapan Teknik Nanopresipitasi pada Nanoenkapsulasi Teofilin dengan Variasi Konsentrasi Polimer dan Surfaktan: Application of Nanoprecipitation Technique on Theophylline Nanoencapsulation with Variation of Polymer Concentration. Jurnal Sains Dan Kesehatan, 5(1), 29–36. https://doi.org/10.25026/jsk.v5i1.1521