Korelasi Kadar Fenol dan Flavonoid terhadap Indeks Aktivitas Antioksidan Ekstrak Batang Vernonia amygdalina
Correlation of Phenol and Flavonoid Content with Antioxidant Activity Index of Vernonia amygdalina Stem Extracts
DOI:
https://doi.org/10.25026/jsk.v5i5.1959Abstract
Bitter leaf (in Africa) is the most widely cultivated species of the genus Vernonia which is the most prominent in the family Asteraceae that has been studied in Africa. African leaves have been widely used for traditional medicine and/or treating various human diseases. This study aimed to determine the bioactive compounds that correlate to the antioxidant activity of V. amygdalina stem. Extraction of V. amygdalina stem was carried out by reflux method. Determination of total phenol and flavonoid content was done spectroscopically. Antioxidant activity was measured based on antioxidant activity index (AAI) using DPPH and CUPRAC methods. Correlation coefficient between bioactive compounds and antioxidant activity using Pearson's correlation method. The results showed the antioxidant activity index of V. amygdalina stem showed strong to very strong activity. The ethyl acetate extract showed the highest total phenol and flavonoid levels. The phenol and flavonoid content of V. amygdalina stem extract showed a strong and significant correlation to the antioxidant activity index with a positive r value
Kata Kunci: AAI, DPPH, CUPRAC, pearson’s correlation
Abstrak
Daun afrika merupakan spesies yang paling banyak dibudidayakan dari genus Vernonia yang paling menonjol dalam famili Asteraceae yang telah dipelajari di Afrika. Daun afrika telah banyak digunakan untuk pengobatan tradisional dan/atau mengobati berbagai penyakit pada manusia. Tujuan dari penelitian ini adalah untuk mengetahui senyawa bioaktif yang berkorelasi terhadap aktivitas antioksidan dari batang V. amygdalina. Ekstraksi batang V. amygdalina dilakukan dengan metode refluks. Penentuan kadar fenol dan flavonoid total dilakukan secara spektroskopi. Aktivitas antioksidan diukur berdasarkan indeks aktivitas antioksidan (AAI) dengan metode DPPH dan CUPRAC. Koefisien korelasi antara senyawa bioaktif dan aktivitas antioksidan menggunakan metode korelasi pearson’s. Hasil penelitian menunjukkan indeks aktivitas antioksidan batang V. amygdalina menunjukkan aktivitas yang kuat hingga sangat kuat. Kadar fenol dan flavonoid total tertinggi ditunjukkan pada ekstrak etil asetat. Kadar fenol dan flavonoid ekstrak batang V. amygdalina menunjukkan korelasi kuat dan signifikan terhadap indeks aktivitas antioksidan dengan nilai r positif.
Kata Kunci: AAI, DPPH, CUPRAC, korelasi pearson’s
References
Kumari T. 2019. A study on knowledge and attitude towards digital health of rural population of india- innovations in practice to improve healthcare in the rural population. International Journal of Emerging Multidisciplinary Research. 3(3):13–21.
Alara OR, Abdurahman NH, Mudalip SKA, dkk. 2017. Phytochemical and pharmacological properties of vernonia amygdalina: a review. 2(1):80–96.
Koubé J, Dongmo SS, Guiama VD, dkk. 2016. Ethnomedicinal survey of Gavdé (Acacia nilotica): a medicinal plant used in sahelian zone of Cameroon, Central Africa. International Journal of Innovation and Applied Studies.16(4):820–827.
Oyeyemi IT, Akinlabi AA, Adewumi A, dkk. 2018. Vernonia amygdalina: A folkloric herb with anthelminthic properties. Beni-Suef University Journal of Basic and Applied Sciences. 7(1):43–49.
Luo X, Jiang Y, Fronczek FR, dkk, 2011. Isolation and structure determination of a sesquiterpene lactone (vernodalinol) from Vernonia amygdalina extracts. Pharm Biol. 49(5):464–470.
Quasie O, Zhang Y-M, Zhang H-J, dkk. 2016. Four new steroid saponins with highly oxidized side chains from the leaves of Vernonia amygdalina. Phytochemistry Letters. 15:16–20.
Owoeye O, Yousuf S, Akhtar M, dkk. 2010. Another anticancer elemanolide from Vernonia amygdalina Del. International Journal of Biological and Chemical Sciences n.d. 4(1):226–234.
Abay SM, Lucantoni L, Dahiya N, et al. 2015. Plasmodium transmission blocking activities of Vernonia amygdalina extracts and isolated compounds. Malaria Journal. 14(1): 288.
Sinisi A, Millán E, Abay SM, dkk. 2015. Poly-Electrophilic Sesquiterpene Lactones from Vernonia amygdalina: New Members and Differences in Their Mechanism of Thiol Trapping and in Bioactivity. J Nat Prod. 78(7):1618–1623.
Farnsworth NR. 1966. Biological and phytochemical screening of plants. J Pharm Sci. 55(3):225–276.
Pourmorad F, Hosseinimehr SJ, Hosseinimehr N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology. 5(11):142–1145.
Chang C, Yang M-H, Wen H-M, dkk. 2020. Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis. 10:178–182.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature. 181(4617):1199–1200.
Apak R, Gorinstein S, Böhm V, dkk. 2013. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry. 85(5):957–998.
Scherer R, Godoy HT. 2009. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chemistry. 112(3):654–658.
Petkova N, Ivanova L, Filova G, dkk. 2017. Antioxidants and carbohydrate content in infusions and microwave extracts from eight medicinal plants. Journal of Applied Pharmaceutical Science. 7(10):55–61.
Dadang J, Irda F, Komar RW, dkk. 2021. Evaluation of xanthine oxidase inhibitory and antioxidant activities of three organs of idat (Cratoxylum glaucum Korth.) and correlation with phytochemical cont. Pharmacognosy Journal. 13(4): 971-976.
Akoglu H. 2018. User’s guide to correlation coefficients. Turk J Emerg Med. 18(3):91–93.
Chen J, Yang J, Ma L, dkk. 2020. Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids. Scientific Reports. 10(1):2611.
Ahmadi SM, Farhoosh R, Sharif A, dkk. 2020. Structure-Antioxidant Activity Relationships of Luteolin and Catechin. J Food Sci. 85(2):298–305.
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