Aktivitas Analgetika dan Antiinflamasi Ekstrak Etanol dan Fraksi Daun Jambu Mawar (Syzygium jambos L.) Secara In Vivo

Analgetic and Anti-inflammatory Activity of Ethanol Extract and Fraction of Guava Leaf (Syzygium jambos L.) In Vivo

  • Deden Winda Suwandi Universitas Garut
  • Tita Puspita Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Garut, Indonesia
  • Doni Anshar Nuari Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Garut, Indonesia
  • Syifa Hamdani Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Garut, Indonesia
Keywords: Syzygium jambos, analgesic, anti-inflammatory, and effective dose.

Abstract

Syzygium jambos is a plant that has traditionally been used to treat several diseases, especially the leaves as a medicine for rheumatism. In this study, the ethanol extract, fraction of n-hexane, ethyl acetate, and water from its leaves were tested for analgesic and anti-inflammatory activity using the writhing method (Siegmund), hot plate, and the formation of swelling of the soles induced by carrageenan lamda. The results showed that the ethanol extract at doses of 100 and 200 mg/kg BW and fractions of n-hexane, ethyl acetate and water at doses of 50 and 100 mg/kg BW had analgesic and anti-inflammatory activities. The ethanol extract at doses of 100 and 200 mg/kg BW can reduce the amount of writhing every 5 minutes for 60 minutes of observation by 84 and 98 times with percent protection of 58.0 and 52.0%. The Ethyl acetate, n-hexane, and water fractions of 50 and 100 mg/kg BW can reduce the amount of animal writhing by 93 and 35 times, 75 and 100 times, as well as 84 and 124 times with the percent of protection is 54.45 and 82.85, 63.0 and 50.9, as well as 58.47 and 38.02%. The analgesic activity of the hot plate method, ethanol extract can increase the survival time of animals in the hot plate by 154.0 and 95.02%, while the animal survival time of the n-hexane fraction is 95.86 and 105.5, ethyl acetate by 143, 7 and 105.7, and the water fraction of 34.1 and 99.5%. The anti-inflammatory activity of ethanol extract at doses of 100 and 200 mg/kg BW was indicated by a decrease in the volume of rat leg edema at the time of observation for 6 hours with the percent inhibition of edema of 59.2 and 41.12%. The n-hexane, ethyl acetate, and water fractions have relatively the same ability, indicated by the edema inhibition value of the n-hexane fraction of 68.24 and 68.56%, the ethyl acetate fraction of 57.08 and 67.32% and the fraction water of 64.02 and 67.46%. The effective dose of extracts and fractions of n-hexane, ethyl acetate, and water as analgesics and anti-inflammatory is 100 mg/kg BW.

 

 

References

[1] Ferrari, F.C. et al. 2016. Effects of Pimenta pseudocaryophyllus extracts on gout: Anti-inflammatory activity and anti-hyperuricemic effect through xantine oxidase and uricosuric action. J Ethnopharmacol.180:37–42.

[2] Jr WAV, Vicentini FTMC, Baracat MM. 2012. Flavonoids as Anti-Inflammatory and Analgesic Drugs : Mechanisms of Action and Perspectives in the Development of Pharmaceutical Forms. 1st ed. Vol. 36, J. Studies in Natural Products Chemistry. Elsevier B.V. 297–330 p.

[3] Sayah K, Chemlal L, Marmouzi I, Jemli M El, Cherrah Y, El M, et al. 2017. South African Journal of Botany In vivo anti-inflammatory and analgesic activities of Cistus salviifolius (L.) and Cistus monspeliensis (L.) aqueous extracts. J. South African J Bot. 113:160–3.

[4] Farrar FC, Darnell L. 2017. Pharmacologic Interventions for Pain Management. J. Crit Care Nurs Clin. 29(4):427–47.

[5] Suwandi, D.W. and Perdana F, 2018. Antihyperuricemia Activity Of Ethanol Extract And Guava Leaf Fractions In Swiss Webster Male. Jurnal Ilmiah Farmako Bahari. 18;35–44.

[6]. Soetomo S, Dizzania N, Martinus A, Choironi NA. 2019. Isolation and Identification of Active Compound from Jambu Mawar Leaves (Syzygium jambos (L.) Alston). J. Sains dan Terapan Kimia. 019;13(2):59–69.

[7] Devakumar J, Keerthana V. 2017. Identification Of Bioactive Compounds By Gas Chromatography-Mass Spectrometry Analysis Of Syzygium Jambos ( L .) Collected From Western Ghats Region Coimbatore, Tamil Nadu. J. Asian journal of Pharmaceutical and Clinical Research. 10(1).

[8] Kurniati NF, Suwandi DW, Yuniati S. 2018. Mukolitic Activity of Combination of Ethanol Extract of Basil Leaves and Ethanol Extract of Red Betel Leaves. J. PSR. 7–13.

[9] Xiao X, Wang X, Gui X, Chen L, Huang B. 2016. Natural flavonoids as promising analgesic candidates: a systematic review. J. Chem Biodivers. 13(11):1427-1440.

[10] Fan S, Ali NA, Basri DF. 2014. Evaluation of Analgesic Activity of the Methanol Extract from the Galls of Quercus infectoria ( Olivier ) in Rats. J. Evidence-Based Complementary and Alternative Medicine. Volume 2014,

[11] Lee CJ, Chen L, Liang W, Wang C. 2010. Anti-inflammatory effects of Punica granatum Linne in vitro and in vivo. J. Food Chem. 118(2):315–22.

[12] Ode W, Zubaydah S, Halik TA. 2018. Anti-Inflammatory Activity Of Pharmaceutical Gel Of Ethanolic Extract From Marine Sponge Xestospongia Sp. J. Borneo journal of Pharmacy. Volume 2(1). P. 1-9.

[13] Gilman, A.G., Rall, T.W. & Nies, A. S., Taylor P. 2012. Goodman and Gilman’s the pharmacological basis of therapeutics. New York: McGraw-Hill.

[14] Sannigrahi S, Knowledge N, Pal D, Ghasidas G. 2015. Flavonoids of Enhydra Fluctuans exhibits analgesic and anti-inflammatory activity in different animal models. J. Pakistan Journal of Pharmaceutical Sciences. 24(3):367-75.

[15] Kumar S, Pandey AK. 2013. Chemistry and Biological Activities of Flavonoids : An Overview. J. Sci World Journal. Volume 2013.

[16] Kumar KA, Kumar MS. 2014. Herbal approach toward Vatarakta (Gout), a Metabolic Syndrome : a review. J. Medicine. 1(2).
Published
2021-04-22
How to Cite
Winda Suwandi, D., Puspita, T., Nuari, D. A., & Hamdani, S. (2021). Aktivitas Analgetika dan Antiinflamasi Ekstrak Etanol dan Fraksi Daun Jambu Mawar (Syzygium jambos L.) Secara In Vivo. Jurnal Sains Dan Kesehatan, 3(2), 218-226. https://doi.org/10.25026/jsk.v3i2.279