Uji Aktivitas Antibakteri Hasil Fermentasi Bakteri Endofit Umbi Talas (Colocasia esculenta L) terhadap Bakteri Pseudomonas aeruginosa

Potential of Antibacterial Compound Fermentation of Endophytic Bacteria from Taro Tuber (Colocasia esculenta L.) againts Pseudomonas aeruginosa

  • Desi Purwaningsih Fakultas Farmasi, Universitas Setia Budi Surakarta
  • Destik Wulandari Fakultas Farmasi, Universitas Setia Budi Surakarta
Keywords: Antibacterial, Endophytic, Colocasia esculenta, Fermentation, Pseudomonas aeruginosa.

Abstract

Bacterial endophyte is an endosimbiont which lives within host plans without causing apparent disease on its host plans. Some endophytic bacteria found in plants have the capability to produce potential compounds as antibacterial. This study aimed to determine the antibacterial activity of the endophytic bacteria fermentation from the tubers of taro plants and determine the optimum time of fermentation which produce the highest antibacterial activity. This research was initially started by producing the suspension of the endophytic bacteria and test bacteria. The suspension that had been made then was used to characterize and identify the endophytic bacteria and test bacteria. Identification methods include colony identification, Gram staining, Spore staining and biochemical tests planted on MCH, SIM, LIA and Citrate mediums. The fermentation was then carried out to produce secondary metabolite compounds which were utilized to examine the antibacterial activity against Pseudomonas auriginosa. The antibacterial activity test was conducted by the disk diffusion assay. The results of the antibacterial activity assay showed that Bacillus siamenssis and Bacillis subtilis were both able to produce secondary metabolites that have potential as antibacterial against Pseudomonas earuginosa. The highest value was achieved by 72 hours fermentation time with a value of 8.7 by Bacillus siamensis. The endophytic bacteria Bacillus subtilis had the highest activity value at 48 hours fermentation time with an average of diameter of inhibition zone of 8.97.

References

[1] S. J. Bhore and G. Sathisha, “Screening of Endophytic Colonizing Bacteria for Cytokinin-Like Compounds : Crude Cell-Free Broth of Endophytic Colonizing Bacteria Is Unsuitable in Cucumber Cotyledon Bioassay,” World J. Agric. Sci., vol. 6, no. 4, pp. 345–352, 2010.
[2] T. Taechowisan, C. Lu, Y. Shen, and S. Lumyong, “Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity,” Microbiology, vol. 151, no. 5, pp. 1691–1695, 2005, doi: 10.1099/mic.0.27758-0.
[3] S. H. Guan, I. Sattler, W. H. Lin, D. A. Guo, and S. Grabley, “p-Aminoacetophenonic acids produced by a mangrove endophyte: Streptomyces griseus subsp.,” J. Nat. Prod., vol. 68, no. 8, pp. 1198–1200, 2005, doi: 10.1021/np0500777.
[4] G. Strobel and B. Daisy, “Bioprospecting for Microbial Endophytes and Their Natural Products,” Microbiol. Mol. Biol. Rev., vol. 67, no. 4, pp. 491–502, 2003, doi: 10.1128/mmbr.67.4.491-502.2003.
[5] P. Simanjuntak, Bustanussalam, D. M. Otovina, M. Rahayuningsih, and E. G. Said, “Studies on endophytic microbes of Artemisia spp. (3). Isolation and identification of artemisinin from product of endophyte microbe cultivation from Artemisia annua,” Maj. Farm. Indones., vol. 15, no. 2, pp. 68–74, 2004.
[6] B. A. Wijaya, G. Citraningtyas, and F. Wehantouw, “Potensi Ekstrak Etanol Tangkai Daun Talas (Colocasia Esculenta [L]) Sebagai Alternatif Obat Luka Pada Kulit Kelinci (Oryctolagus Cuniculus),” Pharmacon, vol. 3, no. 3, pp. 211–219, 2014, doi: 10.35799/pha.3.2014.5409.
[7] K. Assidqi, W. Tjahjaningsih, and S. Sigit, “Potensi ekstrak daun patikan kebo (,” vol. 1, no. 2, pp. 113–124, 2012.
[8] D. Wulandari and D. Purwaningsih, “Uji Aktivitas Antibakteri Ekstrak Etanol Daun Suruhan ( Peperomia pellucida L . Kunth ) Terhadap Bakteri Shigella dysentriae,” J. Farm. Indones., vol. 13, no. 2, pp. 171–177, 2016.
[9] Mukamto, S. Ulfah, W. Mahalina, A. Syauqi, L. Istiqfaroh, and G. Trimulyono, “Mukamto 2015.Pdf.” .
[10] S. A. Brooks, Geo F . Butel, Janet. Morse, Mikrobiologi Iftdokteran, vol. 23. 2004.
[11] M. Poeloengan and Praptiwi, “UJIAKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Gardnia mangostana Linn),” vol. XX, no. 30, pp. 65–69, 2010.
[12] Zohreh Roostan, “Phenanthrene biodegradation by Pseudomonas aeruginosa and Bacillus subtilis isolated from Persian gulf sediments,” African J. Microbiol. Res., vol. 6, no. 21, pp. 4585–4591, 2012, doi: 10.5897/ajmr12.340.
[13] M. F. Imron and I. F. Purwanti, “Uji Kemampuan Bakteri Azotobacter S8 dan Bacillus subtilis untuk Menyisihkan Trivalent Chromium (Cr3+) pada Limbah Cair,” J. Tek. ITS, vol. 5, no. 1, pp. 3–10, 2016, doi: 10.12962/j23373539.v5i1.14854.
[14] A. R. Y. Hibai, Herwin, and R. Kosman, “Antibacterial Activity Assay Of Ethanolic Extract Of Bulbs Sticky Taro (Colocasia esculenta) Use TLC-Bioautografi,” J. Chem. Inf. Model., vol. 07, no. 01, pp. 76–84, 2015.
Published
2021-10-31
How to Cite
Purwaningsih, D., & Wulandari, D. (2021). Uji Aktivitas Antibakteri Hasil Fermentasi Bakteri Endofit Umbi Talas (Colocasia esculenta L) terhadap Bakteri Pseudomonas aeruginosa . Jurnal Sains Dan Kesehatan, 3(5), 750-759. https://doi.org/10.25026/jsk.v3i5.622