Bioaktivitas Ekstrak Etanol Biji Pinang (Arecha catechu L.) terhadap Staphylococcus aureus dan Escherichia coli

Bioactivity of Betel Nut (Arecha catechu L.) Ethanol Extract against Staphylococcus aureus and Escherichia coli

Authors

  • Asrianto Asrianto Poltekkes Kemenkes Jayapura
  • Asrori Asrori Poltekkes Kemenkes Jayapura
  • Indra Taufik Sahli Poltekkes Kemenkes Jayapura
  • Risda Hartati Poltekkes Kemenkes Jayapura
  • Fajar Bakti Kurniawan Poltekkes Kemenkes Jayapura
  • Rina Purwati Poltekkes Kemenkes Jayapura

DOI:

https://doi.org/10.25026/jsk.v3i6.702

Keywords:

areca nut, inhibition, E.coli, S.aureus

Abstract

Areca nut (Areca catechu Linn) is a plant that is widely distributed in tropical and subtropical regions. People have used it to treat various health problems. This study aims to determine the ability of betel nut ethanol extract to inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is experimental. Stages of research, first the betel nut is dried by aerating for 7-10 days. After drying, the seeds are ground into powder. The extraction stage of the active compounds uses the maceration method using 92% ethanol, evaporation is carried out using a rotary vacuum evaporator. The results of the maceration extract were carried out with phytochemical tests and bacterial inhibitory tests against E. coli, and S. aureus, carried out by the Kirby Bauer method. The detected metabolites were flavonoids, tannins and terpenoids. These metabolites have the ability as antimicrobials. The results of the inhibition test of the ethanol extract of areca nut seeds showed descriptively that the ethanol extract had a better inhibitory ability against S. aureus bacteria, compared to E. coli. The conclusion of this study is that the ethanol extract of areca nut seeds can inhibit the growth of E.coli and S.aureus.

References

[1] Salehi, B., Konovalov, D.A., Fru, P. 2020. Areca catechu-from farm to food and biomedical applications. Phytother Res. 34.(9).2140-2158.
[2] Rxlist. 2020. Catechu. Diakses tanggal 21 Juli 2020 melalui laman https://www.rxlist.com/catechu/supplements.htm
[3] Hazarika, D., J. and Sood, K. 2015. In vitro antibacterial activity of peptides isolated from Areca catechu Linn. Der Pharmacia Lettre 7.(1).1-7.
[4] Nurhaida, Usman, F., H, dan Tavita, G., E. 2015. Studi etnobotani tumbuhan obat di Dusun Kelampuk Kecamatan Tanah Pinoh Barat Kabupaten Melawi. Jurnal
Hutan Lestari. 3.(4).526 – 537.
[5] Efremila, Wardenaar, E dan Sisillia, L. 2015. Studi etnobotani tumbuhan obat oleh Etnis Suku Dayak di Desa Kayu Tanam Kecamatan Mandor Kabupaten
Landak. Jurnal Hutan Lestari. 3.(2).234 – 246.
[6] Essien, E., Antia, B., and Etuk, E., I. 2017. Phytoconstituents, antioxidant and antimicrobial activities of Livistona chinensis (Jacquin), Saribus rotundifolius
(Lam.) Blume and Areca catechu Linnaeus Nuts. UK Journal of Pharmaceutical and Biosciences. 5.(1).59 - 67.
[7] Djohari, M., Putri, W., Y., dan Pratiwi, E. 2019. Isolasi dan Uji Aktivitas Daya Hambat Ekstrak Etanol Biji Pinang (Areca catechu L.) Terhadap Bakteri Pada Lidah.
Jurnal Riset Kefarmasian Indonesia. 1.(3). 177-188
[8] Baiti, M., Elrifda, S., dan Lipinwati, L. 2018. Pengaruh pemberian ekstrak etanol biji buah pinang (Areca catechu L.) terhadap pertumbuhan Staphylococus
aureus secara in vitro. JMJ. 6.(1). 10 – 19.
[9] Rajamani, R., Kuppusamy, K., S., Shanmugavadivu, M. and Devadass. 2016. Preliminary phytochemical screening of aqueous extract of Betel Nut and.
International Journal of Biosciense and nanoscience. 3.(1).14-18.
[10] Globinmed. 2020. Areca catechu (L) diakses tanggal 22 Juli 2020 diakses dihttps://www.globinmed.com/index.php?
option=com_content&view=article&id=106303:areca-catechu-l-106303&catid=209&Itemid=143#p12
[11] Ona, W., dan Pongtiku, A. 2016. Buku Tumbuhan Kerarifan Lokal Papua /Papua Traditional Medicine Herbs. Dinas Kesehatan Provinsi Papua. Publikasi
https://www.researchgate.net/publication/321758871.
[12] Amudhan, S., Begum, H., V., and Hebbar, K., B. 2012. A review on phytochemical and pharmacological potential of areca catechuL. Seed. Int J Pharm Sci Res. 3.
(11). 4151- 4157.
[13] Anthikat, R.,R.,N., Michael, A., Kinsalin, V.,A. and Ignacimuthu, S. 2014. Antifungal activity of Areca catechu L. International Journal of Pharmaceutical and
Clinical Science. 4.(1).1-3.
[14] Anthikat, R., R., N. and Michael, A. 2009. Study on the areca nut for its antimicrobial properties. J Young Pharm. 1.(1).42-45.
[15] Rahman, M.,A., Sultana, P., Islam,S., Mahmud, M.,T., Rashid, M.,O. and Hossen, F. 2014. Comparative antimicrobial activity of Areca catechu nut extracts using
different extracting solvents. Bangladesh J Microbiol. 31.(1&2).19-23
[16] Faden, A.,A. 2018. Evaluation of antibacterial activities of aqueous and methanolic extracts of Areca catechu against some opportunistic oral bacteria.
Biosciences Biotechnology Research Asia. 15.(3).655-659
[17] Ramesh, C., H., Mohanraju, R., Kartick, P., and murthy, K., N. 2017. Ef?cacy and effect of Areca catechu nuts. J. Terr. Mar. Res. 1(2).50-53.
[18] Al-Bayati, N., J., M. 2016. In vitro antibacterial and antifungal effect of areca nut extract. Research Journal of Pharmaceutical, Biological and Chemical Sciences,
7.(6).282-286.
[19] Salim, M., Yahya, Sitorus, H., Ni’mah, T., dan Marini. 2016. Hubungan kandungan harah tanah dengan produksi senyawa metabolit sekunder pada tanaman
duku (Lansium domesticum Corr var Duku) dan potensinya sebagai larvasida. Jurnal Vektor Penyakit. 10.(1).11-18.
[20] Sholekha, F,,F. 2017. Perbedaan ketinggian tempat terhadap kandungan flavonoid dan beta karoten buah karika (Carica purbences) daerah Dieng Wonosobo.
Prosiding Seminar Nasional Pendidikan Biologi dan Biologi. Yogyakarta
[21] Xiao, X., Wang, F., Yuan, Y., Liu, J., Liu, Y., Yi, X. 2019. Antibacterial activity and mode of action of dihydromyricetin from Ampelopsis grossedentata leaves
against food-borne bacteria. Molecules. 24. (15). 2831.
[22] Yang, W.,Q., Wang, H.,C., Wang, W.,J.,Wang, Y., Zhang, X.,Q., and Ye, W.,C. 2012. Chemical constituents from the fruits of Areca catechu. Journal of Chinese
Medical Materials. 53.(3).400-403.
[23] Zhang, X., Mei, W., Zeng, Y., and Liu, J. 2009. Phenolic constituents from the fruits of Areca catechu and their antibacterial activities. Journal of Tropical and
Subtropical Botany. 17.(1).74-79.
[24] Ikigai, H., Nakae, T., Hara, Y., Shimamura, T. 1993. Bactericidal catechins damage the lipid bilayer. Biochimica et Biophysica Acta. 1147.(1).132–136.
[25] Chauhan, A., K., Kim, J., Lee, Y., Balasubramaniani, P., K., and Kim, Y. 2019. Isorhamnetin potential for the treatment of Escherichia coli induced sepsis.
Molecules, 24.(21). Doi:10.3390/molecules24213984.
[26] Ren, X., Bao, Y., Zhu, Y., Liu, S., Peng, Z., Zhang, Y. and Zhou, G. 2019. Isorhamnetin, hispidulim and cirsimaritin identified in Tamarix ramasissima barks from
Southern Xinjian and their antioxidant and antimicrobial activities. Molecules. 24.(3).390.Doi: 10.3390/molecules24030390.
[27] Jnawali, H., Jeon, D., Jeong, M., et al. 2016.Antituberculosis activity of a naturally occurring flavonoid, isorhamnetin. J Nat Prod. 79.(4).961-9.
[28] Bhattacharya, D., Ghosh, D., Bhattacharya, S., Sarkar, S., Karmakar, P., Koley, H., Gachhui, R. 2018. Antibacterial activity of polyphenolic fraction of Kombucha
against Vibrio cholerae: Targeting cell membrane, Lett. Appl. Microbiol. 66.(2).145–152, https://doi.org/10.1111/lam.12829.
[29] Habtamu, A, and Melaku, Y. 2018. Antibacterial and antioxidant compounds from the flower extracts of Vernonia amygdalina, Adv. Pharmacol.
Sci.https://doi.org/10.1155/2018/4083736.
[30] Zhang, T. Qiu, Y., Luo, Q., Zhao, L., Yan, X., Ding, Q., Jiang, H., Yang, H. 2018. The mechanism by which luteolin distrips the cytoplasmic membrane of methicillin
resistant Staphylococcus aureus. J.Phys. Chem.B. 122 : 1427-1438.
[31] Lv, P.,C., Li, H.,Q., Xue J.,Y., Shi L., Zhu, H.,L. 2009. Synthesis and biological evaluation of novel luteolin derivatives as antibacterial agents. Eur J Med Chem. 44.
(2).908–914.
[32] Qian, W., Liu, M., Fu, Y., et al. Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties.
Microb Pathogens. 142:.104056. doi:10.1016/j.micpath.2020.104056.
[33] Wang, Q., Xie, M., J. 2010. Antibacterial activity and mechanism of Luteolin on Staphylococcus aureus. Acta Microbiol Sin. 50.(9).1180–1184.
[34] Plaper, A., Golob,M., Bratkovic, I., V., Oblok, M. 2003. Characterization of quercetin binding site on DNA gyrase. Biochemical and Biophysical Research
Communication. 306.(2).530-536
[35] Yang, D., Wang, T., Long, M., Li, P. 2020. Quercetin: Its main pharmacological activity and potential application in clinical medicine. Oxidative medicine and
Celuller Longevity. https://doi.org/10.1155/2020/8825387
[36] Ansari, A., Mahmood, T., Bagga, P., Ahsan, S., Shamim, A., Ahmad, S., Shariq, M., Parveen, S. 2021. Areca catechu: A phytopharmacological legwork. Food
Frontiers. 2.(2).163-183.
[37] Nonaka, G. I., Hsu, F. L., and Nishioka, I. 1981. Structures of dimeric, trimeric, and tetrameric procyanidins from Areca catechu L. Journal of the Chemical
Society, Chemical Communications, (15), 781– 783
[38] Peng, W., Liu, Y., Wu, N., Sun, T., He, X., and Gao, Y. 2015. Areca catechu L.(Arecaceae): A review of its traditional uses, botany, phytochemistry, pharmacology
and toxicology. Journal of Ethnopharmacology. 164. 340- 356.
[39] Wu, M. and Brown, A.,C. 2021. Application of catechins in the treatment of bacterial infections. Pathogens. 10. https://doi.org/10.3390/pathogens10050546
[40] Hoshino, N.,Kimura, T.,Yamaji, A., and Ando, T. 1999. Damage to the cytoplasmicmembrane of Eschericia coli by catechin-copper(II) complexes. Free Radic Biol
Med. 27. 1245-1250

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Published

2021-12-31

How to Cite

Asrianto, A., Asrori, A., Sahli, I. T., Hartati, R., Kurniawan, F. B., & Purwati, R. (2021). Bioaktivitas Ekstrak Etanol Biji Pinang (Arecha catechu L.) terhadap Staphylococcus aureus dan Escherichia coli: Bioactivity of Betel Nut (Arecha catechu L.) Ethanol Extract against Staphylococcus aureus and Escherichia coli. Jurnal Sains Dan Kesehatan, 3(6), 839–845. https://doi.org/10.25026/jsk.v3i6.702